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Information × Registration Number 0218U001552, 0116U002409 , R & D reports Title To develop and justify the feasibility of modern nano- and cellular technologies to enhance the anticancer and immunomodulatory properties of vaccines based on dendritic cells popup.stage_title Head Khranovska Natalia, Registration Date 22-02-2018 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 The object of the study is the phenotypic and functional characteristics of dendritic cells (DCs) loaded with iron oxide nanocomplexes or PLGA nanoparticles. The aim of the work is to develop the technologies for the production of dendritic cells loaded with nanocomplexes or liposomes containing tumor antigens and to investigate their biological properties. Methods - methods of experimental oncology, cell culture method, cytofluorometric, immunological, molecular genetic, statistical methods. Equipment - FACSCalibur flow cytofluorometer, OPN-3 centrifuges, microscope, WPS 600 / C and BT scales, AxioZeizz, Germany, Imager.M1 fluorescence microscope, Nano Drop 1000 spectrophotometer, 7500 Real-Time PCR Systems. The technology of obtaining a magnetic nanocomplex (MNC) was developed, it includes the mechanical modification of freeze-dried tumor cells and nanoparticles (NP) of Fe3O4 iron oxide in MMVE-0.005 microvibroline. The method of obtaining cytological samples of the generated DCs loaded with MNCs was developed with the modified Lille method. The results of cytological studies have shown that 100% of DCs had the ability to absorb iron oxide NPs, and that the absorption capacity of DCs in healthy people and cancer patients did not differ. The most optimal concentration of iron oxide NP for the loading of generated DC was 8*10-12 g/ml. Exposure of the DC with NP during 24 hours did not affect their viability, in particular the level of apoptosis, the distribution of cells in the cell cycle phases, and the number of devitalized cells in the culture. A new antitumor vaccine based on mouse DCs and MNC using a Lewis lung carcinoma cells was created. The experiment showed that in mice with Lewis lung carcinoma, intracutaneous administration of DC-based vaccine and magnetic field exposure were accompanied by the most pronounced antimetastatic effect, which was manifested in a significant reduction in the number of metastases in the lungs of the mice compared to control groups and some decrease in the mass of the primary tumor due to a significant reduction of the number of S-phase cells in the tumor. The combined effect of DC vaccine and magnetic field contributed to a significant decrease in the level of IL-4 mRNA in the spleen and some increase in the level of IFN-? mRNA, which may indicate polarization of the Th-1-type immune response. Combination therapy based on DC vaccine and magnetic field helped to reduce the expression of TGF-? mRNA and FoxP3 transcription factor in the spleen and lymph nodes, which can be considered as one of the mechanisms that provides the implementation of antitumor therapy effect. The technology for obtaining of NPs from copolymers of milk and glycolic acids (PLGA) and lysate of tumor cells was developed. It has been found that PLGA-NPs had a size from 230 to 370nm. Product Description popup.authors Іномістова М.В. Скачкова О.В. Храновська Н.М. Шумейко І. popup.nrat_date 2020-04-02 Close