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Information × Registration Number 0218U005112, 0116U002209 , R & D reports Title Genetic and biochemical aspects of the regulation of some catabolic and anabolic processes in microorganisms: alcoholic fermentation, catabolism of methanol, biosynthesis of flavins, glycerol, hydrogen and glutathione popup.stage_title Head Sibirny Andriy Andrijovych, Registration Date 01-03-2018 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The method of editing CRISPR / Cas9 genome for yeast Candida famata was developed. The additional modification of the CRISPR / Cas9 system was in the expression of the CAS9 gene under the control of the maltose-regulated promoter of the CfMAL2 gene.The conserved transcription factor Tup1 of the yeast Pichia guilliermondii has been identified. It was found that Tup1 is involved in regulation of biosynthesis of riboflavin and transport of iron into cells. SFU1 C. famata gene amplified and sequenced, Cassette for deletion of the SFU1 gene were constructed and will be used for transformation in the recipient of C. famata strains. Some plasmids containing RIB1, RIB6 and RIB7 genes of riboflavin biosynthesis encoding for GTP cyclohydrolase II, 3,4-dihydroxy-2-butanone-4-phosphatase and 3-dihydroxy-2-butanone-4-phosphatase synthase and riboflavin synthase, respectively, were constructed to detect limiting parts of riboflavin.biosynthesis. It has been shown that the combination of RIB1 and RIB6 genes exhibits the highest positive effect on riboflavin production.To construct of aminoriboflavin yeast producer, the modified rosB gene Streptomyces davawensis (encoding N, N-8-demethyl-8-aminoriboflavin-5'-phosphate synthetase, the key gene of biosynthesis of aminoriboflavin) has been expresed in the C.famata strain able to over production of flavin mononucleotide Recombinant strains that are capable of producing an additional fluorescing product, probably aminoriboflavin, have been obtained. Product Description popup.authors Булботка Н. Василишин Р. Дмитрук К.В. Куриленко О.О. Левків К.О. Петровська Я. Семків М.В. Фаюра Л.Р. Федоренко В.О. Федорович Д.В. Цирульник А.О. Юрків М. popup.nrat_date 2020-04-02 Close