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Information × Registration Number 0218U005291, 0116U000039 , R & D reports Title Development of cell test system to evaluate the efficacy and mechanisms of action of antineoplastic drugs. popup.stage_title Head Sydorenko Myhail V., Registration Date 16-08-2018 Organization "Department of Biotechnical Problems in Diagnostic of Institute of Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine" popup.description2 Abstract. Research report: p. 48, Fig. - 12, Table 1, reference sources – 49. The object of research: tumor cell line MCF-7 in a monolayer and spheroid growth, cell model multicellular tumor spheroids. Methods: Tumor cell culture methods MTT assay, light microscopy, cytofluorometry. The study aims to improve and standardize the test cellular system based protocols multicellular tumor spheroids of cell lines of epithelial origin to determine the efficacy and mechanisms of action of antineoplastic drugs like those that are used clinically, and undergoing preclinical testing. The objectives of the study was to establish the optimal conditions for culturing tumor cells of breast cancer MCF-7 for creating randomized spheroids and characteristics and parameters of the system for the МTS line MCF-7. As a result, protocols for generating MCF-7 line were developed МTS and compared the reactivity of tumor cells when cultured in a monolayer system and spheroid growth. Also, it was determined that on day 4 МTS volume increased to 2.1 times the percentage necrotic area was 37% of the overall diameter of the МTS. At 10% ETC conditions (fetal calf serum) in complete medium of incubation revealed the highest proliferative activity of MCF-7 cells. Percentage of dead cells under incubation with 10% ETS does not exceed 10%. TAM reduced the survival of tumor cells in 2D culture for 80%, and in spheroid cultures (3D) - 23%. IFN? had no significant effect on the 3D culture simultaneously reduced cell survival in 2D culture for 15%. At the same time, K medium from stimulated T-lymphocytes 3D culture growth by 40%, and 2D culture inhibited by 58%. A combined effect of IFN? and IFN? and estradiol and tamoxifen had a cytotoxic effect on the credible monolayer culture (48% and 80%, respectively) and had no significant effect on spheroid culture. Effect of tamoxifen as single or in combination with estradiol and K medium from T-lymphocytes have a pronounced cytotoxic effect on a 2D culture. In the 3D cultures of these substances do not have such an effect. By the environment of T-lymphocytes also had a cytotoxic effect on the 2D culture, as a separate component and in combination with estradiol or tamoxifen. Thus in 3D culture, on the contrary, increased the percentage of living cells: in the presence of K-medium - up to 140% compared with the control, in the presence of K-medium and estradiol - up to 117%. It has also been demonstrated that tumor cells in the 3D culture less sensitive to disturbing signals and antiproliferatsionnym they share continued antiproliferative effects even with tamoxifen. Simultaneously, there was a decrease in the percent cells S-phase at all conditions in Experiment 3D cultures compared to 2D. Product Description popup.authors Гергелюк Тетяна Сергіївна Железна М.В. Лебедєва Т.М. Нестеренко О.М. Оцалюк В.М. Перепелиціна Олена Михайлівна Сидоренко Михайло Васильвич Юрченко Н.П. Ястребова Олена Вікторівна popup.nrat_date 2020-04-02 Close