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Information × Registration Number 0219U000900, 0117U004740 , R & D reports Title New polymeric composite materials with complex enzymatic activity, perspective for use in therapy of wounds and burns in military field conditions and medicine of catastrophes popup.stage_title Head Romanovska Irina Igorivna, Доктор біологічних наук Registration Date 14-03-2019 Organization A.V. Bogatsky, Physico-Chemical Institute of the National Academy of Sciences of Ukraine popup.description2 The object of investigation - proteinase of Bacillus thuringiensis with collagenolytic, elasolytic and proteolytic activities. The purpose of the study is to create new composite polymeric materials of prolonged complex proteolytic action based on chitosan, polyvinyl alcohol, cellulose and its derivatives with the new enzyme of Bacillus thuringiensis to study their biochemical and physicochemical properties. Isolated protease of Bacillus thuringiensis var. israelensis IMB B-7465 with M.m. 34 kDa. As a result of immobilization of the enzyme into the polyvinyl alcohol / chitosan matrix, a preparation with complex proteolytic activity was obtained. Immobilized protease is 32-35% more stable in alkaline and acidic media and retains its original caseinolytic activity for 12 months at 4 °C. The kinetics of casein hydrolysis by free protease was studied, and a comparative analysis of methods for linearizing the Michaelis-Menten equation using the various methods for determining activity was carried out. It has been established that the method for determining caseinolytic activity using o-phthalic dialdehyde makes it possible to determine the NH2-group of the products of hydrolysis of a protein substrate by the number of peptide bonds broken, that is, it is most likely to detect the depth of proteolysis by a free enzyme. Substrate inhibition of free protease activity was detected (Ki 1.30-1.71 g/dm3). It is shown, that the inclusion of the enzyme in the matrix does not significantly affect the Vmax. hydrolysis; Km increases 1,4-2,3 fold, depending on the method of linearization; in the case of an immobilized enzyme, Ki is almost three times as high as the free one. Product Description popup.authors Є.А. Шестеренко І. І. Романовська Л.Д. Варбанець Н.А. Дзюблюк О.В. Севастьянов Рижак А.А. Ю.А. Шестеренко popup.nrat_date 2020-04-02 Close