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Information × Registration Number 0219U001304, 0117U004313 , R & D reports Title Investigation of the ability to biofilm formation and pathogenic properties of pathogens of infectious-inflammatory complications in patients with acute leukemia and determination of the role of probiotic drugs in the destruction of microbial biofilms popup.stage_title Head Rybalska A.P., Registration Date 23-01-2019 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Object of study: 15 patients with AML and ALL, 207 strains of microorganisms. Research methods: laboratory-clinical, hematological, immunocytochemical, microbiological, immunological. Results. The effect of a domestic probiotic preparation containing bifidobacteria, lactobacilli, propionibacteria, streptococcus thermophilus was studied on hematological parameters, the immune status of patients, the microecology of the biotopes of the upper respiratory tract and intestines, the properties of microorganisms - adhesive potential and the ability to form biofilms. It has been shown that probiotic does not affect on the hematopoietic system of the patients, however, it helps to restore the intestinal microflora by increasing bifidobacteria (34.7% of patients), lactobacilli (30.0% of patients) and eliminating conditionally pathogenic cultures from biotopes. In the biotopes of the nasal cavity and throat of the patients, "latent pathogens" were identified - microorganisms that were not isolated at the beginning of the study, but were isolated in clinically significant titers at the end of probiotic intake. These are strains of the genus Staphylococcus, Micrococcus and Moraxella catarrhalis, mycelial and yeast-like fungi of the genera Aspergillus, Candida, which made it possible to more thoroughly characterize the microbiocenosis of biotopes and may have an additional diagnostic effect. Staphylococcus aureus, S. epidermidis and S. saprophyticus were found to have a high (42.86% of strains) and a moderate (57.14% of strains) degree of adhesion, as well as a high level of biofilm formation (0.319-1.329 optical density units using the ELISA method). The use of bacterial symbiotics positively influenced on S.epidermidis cultures, reducing their pathogenic potential - the number of strains synthesizing hemolysin, phosphatase, fibrinolysin and lysozyme decreased by 8.2%, 10.0%, 17.4% and 30.6%, respectively. This contributed to the reduction of colonization resistance of these microorganisms or their elimination. The use of a probiotic preparation contributed to the normalization of the population and subpopulation composition of peripheral blood lymphocytes and stimulation of the phagocytic immunity of patients indicators of the final stage of phagocytosis increased by 17.7% (p <0.05). Thus, the use of probiotics in patients with acute leukemia had a positive effect on eliminating dysbiotic states during immunosuppression and, as a result, prevented the occurrence of infectious and inflammatory complications, allowing the treatment of the underlying disease in full. Medicine. Product Description popup.authors Мазур М.М. Мельник О.А. Немировська Л.М. Скачкова Н.К. Третяк Н.М. popup.nrat_date 2020-04-02 Close