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Information × Registration Number 0219U001693, 0115U004187 , R & D reports Title Using molecular and cellular technologies for biotech wheat and maize plants resistant to the herbicide glyphosate obtaining popup.stage_title Head Morgun Bogdan Volodymyrovych, Registration Date 18-02-2019 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The Agrobacterium-mediated transformation of 18 day-old callus was carried out on obtained from apical meristem of wheat cultivars Podolianka, Zimoyarka and Yatran 60 (595 pieces of primary callus of wheat cultivar Zimoyarka, 548 pieces of cultivar Podolianka and 448 pieces of cultivar Yatran 60), with genetic vectors, the T-DNA of which contained genes CP4 epsps (resistance to glyphosate) and nptII (kanamycin resistance). As a result, four transforms of the Podolanka cultivar were obtained. The Agrobacterium-mediated transformation of 1791 calli obtained from immature wheat embryos of the Podolanka variety was carried out with the same vectors. After selection, 144 regenerant plants were chosen, 45 of which were analyzed by PCR-method. The sequence of nptII gene was detected in 17 plants. The vector pCB135 and the regenerative-active genotype PLS61xDK267 have been selected for biolistic transformation of maize callus. It was found that the use of maize callus of hybrid genotypes for genetic transformation was more effective compared to callus of the inbred lines. As a result of Agrobacterium-mediated transformation of 1731 calli, obtained from immature embryos of maize, 18 potential transformant plants were regenerated. The work has been carried out to develop a vector with glyphosate oxidase gene. Product Description popup.authors Банникова Марія Олександрівна Жалій Наталія Андріївна Нітовська Ірина Олександрівна popup.nrat_date 2020-04-02 Close