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Information × Registration Number 0219U001827, 0118U006260 , R & D reports Title Development and optimization of enzyme-based electrochemical biosensors on the basis of new functional nanomaterials for the determination of arginine and their application for quality control of fruit juices and hygiene products. Section 3. Development of reference methods for the arginine analysis with the usage of arginine-hydrolyzing recombinant enzymes. popup.stage_title Head Sibirny Andriy, Registration Date 06-03-2019 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The goal of the comprehensive Project: design and optimization of enzymatic electrochemical biosensors based on new functional nanomaterials for determination of arginine and its application to quality control of fruit and Berry juices, as well as hygiene products. The purpose of part 3 of the Project: development of reference methods of analysis of arginine for the use of arginine-hydrolizing recombinant enzymes. The aim of phase 1 (2018), part 3 of the Project: the development of enzimatic-chemical method of arginine analysis using arginase and testing it on real samples of fruit juices. Research methods: microbiological, biochemical, physico-chemical, analytical. As a result of the work in 2018: arginine-selective recombinant enzymes, namely, arginase and arginine deiminase from the corresponding microbial cells - over-producers of these enzymes, were isolated and characterized; enzymatic method of L-arginine analysis, based on arginase and acetylmonooxim, with spectrophotometric mode of signal detection was developed; this method have been successfully used for arginine analysis in real samples of fruit juices, thus it will be promising for exact and selective assay of arginine in beverages. The proposed scientific research corresponds to the level of the best foreign prototypes. On the base of scientific results, 6 theses were published, 3 articles are in press, patent of Ukraine is under revision now. Product Description popup.authors Іваш М.Ф. Гайда Г.З. Куцяба В.І. Фаюра Л.Р. popup.nrat_date 2020-04-02 Close