Registration Number
0219U003254,
0116U000367
, R & D reports
Title
Study of the influence of neuronal stem cells on the morphofunctional state of the inner ear of experimental animals in experimentally induced sensoneurological deafness
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Head
Timen Grigoriy E., Доктор медичних наук
Registration Date
30-01-2019
Organization
Kiyv Research Institute of Otolaryngology named after prof. O.S.Kolomiychenko
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A sub-cluster approach for the introduction of neuronal stem cells into the inner ear of laboratory animals has been developed. It has been shown that intramuscular, daily administration of gentamicin to guinea pigs for 14 days, at a dose of 100 mg / kg weight, resulted in sensoneural deafness or deafness, which is confirmed by the data of short-headed auditory-induced potentials and otoacoustic emission . The use of the model of sensorineural hearing loss caused by the action of a mixture of gentamicin and dimethoxide results in immediate hearing loss without causing general toxic manifestations of an aminoglycoside antibiotic. Intravenous, intracavitary, and suboccitalial administration of neuronal stem cells in 14 days after the experimentally received SDP, led to the normalization of auditory function in animals, which was confirmed as objective (short-acting auditory-induced potentials and otoacoustic emission) by survey methods. For the first time it was discovered that subccipital injection of neuronal stem cells makes it possible, bypassing the hematoencephalic and hematolabirint barriers, to deliver them directly to the body of Kortiev. The method of obtaining embryonic neuronal cells of the primary culture of tissue, modified by us, has demonstrated the possibility of its further use in experimental otorhinolaryngology.
Product Description
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Бєлоусова Алла Олександрівна
Вінничук Павло Васильович
Кривохатська Людмила Дмитрівна
Руденька Катерина Леонідівна
Сапіжак Ірина Ігорівна
Чернишова Наталя Констянтинівна
Чубко Сергій Петрович
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2020-04-02
