Registration Number
0219U006209,
0117U002580
, R & D reports
Title
Early molecular genetic and immunohistochemical predicting of susceptibility to lung and digestive system cancer progression.
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Head
Tumanskiy Valeriі Оleksііоvуch,
Registration Date
24-04-2019
Organization
Zaporozhye State Medical University
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It was determined that colorectal adenocarcinoma (CRA), compared with unchanged mucous membrane of the distal parts of the colon, is characterized by reduced transcriptional activity of the CDH1 and low expression of E-cadherin, as well as elevated transcriptional the activity of the CTNNB1 gene and the high level of B-catenin expression. In CRA cells have direct correlation between levels of expression the genes studied and molecules encoded by them. When the CRA progresses from I to IV stage it has a tendency to decrease in tumor cells the level of expression of the mRNA of the CDH1 gene and the reduction of the expression level of E-cadherin. The statistically significant difference between the expression levels of the mRNA of the CTNNB1 gene and the B-catenin in tumor cells when progressing CRA is not It was found that with the progression of colorectal adenocarcinoma from stage I to IV in tumor cells increases transcriptional the activity of the K-RAS gene and the transcriptional activity of the gene reduced Ki-67. Tumor cells of the KRA are characterized by an average level proliferation (by expression Ki-67), while there is a reverse the correlation between the Ki-67 expression level and the depth index invasion pT cancer. With the progression of CRA, transcription increases the activity of the TP53 gene, which correlates with increased transcriptional activity the activity of the K-RAS gene. Tumor cells of the CRA are characterized by a mean level of expression of the oncoprotein p53 and low marker expression apoptotic degradation of caspase-3, established the inverse correlation between an elevated level of transcription activity of the K-RAS gene and a low level apoptosis of KRA tumor cells. There are back correlations between levels of KRAS and CDH1 gene mRNA expression, KRAS gene mRNA and expression E-cadherin, as well as a direct relationship between the expression levels of the mRNA of the KRAS gene and expression of B-catenin.
Product Description
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Баударбекова М.М
Бортніков Ю.М.
Камишний О.М.
Пірогова З.О.
Христенко Т.О.
Шишкін М.А.
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2020-04-02
