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Information × Registration Number 0219U100671, 0113U004305 , R & D reports Title The role of different forms of Bcr-Abl protein in the signaling pathway and the formation of tumor phenotype in myeloproliferative neoplasms. popup.stage_title Head Telegeev Gennadiy D., Доктор біологічних наук Registration Date 22-02-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The research is devoted to the study of the molecular pathogenesis of Ph-positive leukemias, the development of diagnostic and monitoring methods for monitoring myeloproliferative neoplasms, approaches to therapy.The standard molecular-biological protocols were used, including: RNA and DNA isolation, selection of oligonucleotide primers, reverse transcriptase polymerase chain reaction (PCT), PCR, real-time PCR, recombinant DNA generation, recombinant protein synthesis, bacteria transformation , isolation and restriction analysis of plasmid DNA, Southern hybridization, DNA sequencing, sequential analysis, affinity chromatography, eukaryotic cell transformation, Western blot analysis, fluorescence microscopy, l blood culture cells, microscopy of ultra high resolution STED. Based on the data of mass spectrometry, the interaction of the Bcr PH domain with 23 proteins was studied.Recombinant vectors carrying the DNA sequence of these target proteins for their bacterial and eukaryotic expression was received. Protein binding has been confirmed for: SMC1, β-tubulin, clathrin, Usp 1, GLG1, cortatin. It has been shown that the USP1 inhibitor (ML323) leads to a change in the cellular localization of USP1. A MN-multi-system-system for the detection of mutations in patients with myeloid neoplasms has been proposed. Immunofluorescent analysis revealed colocalisation of the GLG1 protein with the Bcr-Abl protein kinase in the K562 cells. By the results of coimmunoprecipitation, the interaction of the GLG1 protein and the Bcr-Abl T cell protein in the K562 cells was established. Product Description popup.authors popup.nrat_date 2020-04-02 Close