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Information × Registration Number 0219U100949, 0118U003663 , R & D reports Title Study of molecular biological and phenotypic manifestation of transmitted genes performance and their inheritance in biotechnological plants popup.stage_title Head Morgun Bogdan V., Кандидат біологічних наук Registration Date 05-04-2019 Organization Institute of Cell Biology and Genetic Engineering popup.description2  Agrobacterium-mediated transformation of wheat plants of the Podolyanka cultivar by constructions pCB135, p12 and p13 containing npt II and CP4 epsps genes was carried out. The T1 plants, according to the PCR, contained the sequence of the gene npt II, were selected. The analysis of wheat T1 plants obtained as a result of Agrobacterium-mediated transformation in planta (2017) by constructs pCB133, pCB135, pCB182 and pCB241; It was established that transgenic plants were obtained only using the pCB135 construct, with the T-DNA of the pCB135 constructs only partially embedded in the wheat genome. Inheritance of the npt II transgenes in T2 plants, obtained as a result of self-pollination, did not occur. After the biological transformation of maize genotypes PLS61 and PLS61 × DK633 / 266, T1 and T2 generations were obtained, which according to the PCR results contained the sequence of the bar-gene. Agrobacterium-mediated transformation of maize plants in planta with vector pCB133 gave seven T1 plants containing bar gene, but all of them were sensitive to Basta herbicide. The environments for callusogenesys and regeneration of spelt wheat were selected; rooted regenerants were produced from immature embryos, which were transferred in vivo. The study of rho0 mutants (loss of mitochondrial DNA) SK1p of the yeast strain Saccharomyces cerevisiae was carried; Stress caused by fasting led to a significant complication of the structure of SK1 strains on biofilms and their fragmentation. The promotor sequences of transcription factor TaWRKY2 of wheat cultivars Darunok Podillya and Polisska 90 were aligned; One SNP was found in the Darunok Podillya and 3 inserts, 3 deletions and 17 SNPs in the Poliska 90. Measuring the transcription level of flavonoid biosynthesis genes by real-time PCR method was carried out and found that genetic transformation led to significant changes in the activity of the chs and pal genes of Artemisia tilesii.  Product Description popup.authors Bannikova Maria О. Nitovska Iryna O. Pronina Olha Volodymyrivna popup.nrat_date 2020-04-02 Close