Registration Number
0219U100962,
0117U006276
, R & D reports
Title
Development of new generation implants is based on synthetic bone tissue analogues and mesenchymal stem cells for bone tissue damage reconstruction.
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Head
Piven Oksana O., Кандидат біологічних наук
Registration Date
10-04-2019
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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During the implementation of the scientific and technical project, according to the schedule, we have optimized the cultivation conditions of the mesenchymal stem cells (MSCs) of the rat isolated from adipose tissue on granules of bioactive ceramics of different composition, size and geometry. Using the methods of cellular biology we analyzed the growth characteristics of cells, their metabolic activity and morphology in the system of cultivation with bioactive ceramics, which resulted in the selection of the best composites for application on them of cells: Biomin GT1 - H2O; Biomin GT-500 - H2O; Biomin GTalk 700 (800 ° C) - H2O; Biomin GTalk 700 (850 ° C) - H2O. A method for cultivating MSCs on bioactive ceramic granules was developed. In addition, using the FACS method, we carried out immunophenotyping of MSCs obtained from rat bone marrow and adipose tissue. It is shown that by the level of representation of surface markers typical for MSCs, the cells we receive correspond to the profile typical for MSCs. Also during the reporting year, we developed a model of lesion of bone tissue of the rat, which will be used during the next stage. According to the main results of the research the publication is being prepared, the Project became the finalist of the Sikorsky Challenge 2018 Startup Competition
Product Description
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Balatskyi Volodymyr V.
Matsevych Larysa L.
Ruban Tetyana A.
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2020-04-02
