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Registration Number

0219U101463, 0117U001929 , R & D reports

Title

Making of new dermal wound coverings for treatment of massive burns and traumatic skin lesions with other genesis

Head

Lukash Lyubov Leonidivna, Доктор біологічних наук

Registration Date

05-08-2019

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

Description

During the reporting period, experimental samples of dermal coverings were developed using: a) gelators of natural and artificial origin; b) human cells of three different lines; c) cell-free and cells conditioned medium (CFCCM). Two cell lines, 4BL mesenchymal stem cells and E-8 germinal cells of embryonic origin were obtained by the authors of the project, and the line of A-102 skin fibroblasts was courtesy given by Professor McCormick, USA. Cryopreserved aliquots of cellular materials and CFCCM have been prepared for experiments and prolonged storage. The technology of obtaining soft gel mixtures with the inclusion of cellular components or their derivatives and the method of formation of burns of degree IIIb using experimental animals (mice and rats) have been developed. In the study of the therapeutic effect of the studied drugs found that statistically significant accelerated healing of burn wounds in mice was observed with the use of gel mixtures with the inclusion of cells 4BL and A102. The most effective among the studied CFCCM was a drug based on the culture medium, conditioned by 4BL cells.

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Kushniruk Veronika Olegivna
Macewicz Larysa
Papuga Alexander
Ruban Tatiana