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Information × Registration Number 0219U102022, 0119U101914 , R & D reports Title The effect of circulating viral transactivator proteins (HIV-1 Tat and EBV Zta) on gene expression in B-cell lymphomagenesis popup.stage_title Head Skrypkina Inessa Ya., Кандидат біологічних наук Registration Date 13-12-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  Using the generated recombinant constructs containing the complete coding sequences of the ERVW-1 gene in the newly created pCDH-Puro vector for lentiviral transduction, and the constructs with four integrated guide pins in the pSpCas9(BB)-A2-Puro vector for CRISPR/Cas9 to create knockout ERVW-1 lines of B cells and astrocyte cells with ectopic expression of this gene. We selected 26 mutant knockout cell lines for ERVW-1 gene knockout mutations and identified with ectopic expression of this gene Product Description popup.authors Anopriyenko Olga V. Areshkov Pavlo O. Shablii Volodymyr A. popup.nrat_date 2020-04-02 Close