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Information × Registration Number 0219U102119, 0117U001792 , R & D reports Title Creation of means for detection of medical markers based on fluorescently labeled monoclonal antibodies popup.stage_title Head Shlapatska Larysa M., Кандидат біологічних наук Registration Date 19-12-2019 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  Approaches have been developed to obtain bright conjugates with fluorescent protein PerCP, tandem dye PC5 and its domestic counterpart R-PE-ISC-650 (R-PE-ISC-Cy5). The preparation of the conjugates was tested on immunoglobulin G (IgG) and included modification of IgG 2-iminothiolane and modification of PerCP or tandem dyes by succinimidyl-4- (N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) conoxylate (SMCC) fluorescence labeled antibody (IgG) antibody - PC5, R-PE-ISC-650 or PerCP. It was found that increasing the number of cross-linkers used in protein modification leads to the formation of unwanted fluorescent labeled IgG aggregates and insoluble precipitate. The decrease in the number of cross-linkers used results in only partial labeling of antibodies or the formation of IgG conjugates with a small number of fluorescent label molecules. The fluorescence of all the conjugates obtained is well excited by a 488 nm laser, but in the case of labeling with antibodies by tandem dyes (PC5, R-PE-ISC-650) for use in cytofluorimetric studies, it is necessary to obtain the conjugate at a ratio of 1: 1 to tandem dye, whereas to obtain a similar signal for conjugates with PerCP it is necessary to obtain a conjugate in the ratio of 1: 4-5 the number of antibodies to PerCP. Product Description popup.authors Kobzev Dmytro V. Tatarets Anatolii L. Shlapatska Larysa M. Scherbina Valeriia M. popup.nrat_date 2020-04-02 Close