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Information × Registration Number 0219U102356, 0119U103477 , R & D reports Title Analysis of the effects of interspecific introgressive hybridization based on microsatellites (as exemplified by the Southern meat cattle) popup.stage_title Head Kramarenko Olexander S., Кандидат сільськогосподарських наук Registration Date 27-12-2019 Organization Mykolayiv National Agrarian University popup.description2  The main aim of this work was to study the genetic structure of the Southern Meat breed (SM) cattle and to assess the taurine/zebuine admixture in the SM population using microsatellites. A set of 192 heifers was included in the study. Based on the origin of the individuals studied, all heifers were attributed to two groups according to the different degree of Zebu blood: the LZ group (≤ 3/8 percent Zebu blood; n = 100) and the UZ group (> 3/8 percent Zebu blood; n = 92). Set of autosomal polymorphic microsatellite loci (BM1818, BM1824, BM2113, ETH3, ETH10, INRA023, TGLA53, TGLA122, TGLA227 and SPS115) were genotyped to estimate various parameters of genetic diversity. The total number of genotype estimates ranged substantially over loci from 18 (locus BM1824) to 37 (locus INRA023), giving a mean number of 27.9 ± 1.96 genotypes per locus. Overall, one hundred and four alleles were observed across the microsatellite markers examined, with allelic diversity (the average number of observed alleles per locus) of 10.4 ± 0.76. At each locus some alleles were identified that were present at higher frequencies in the LZ group and absent or present at relatively lower frequencies in the UZ group, or vice versa. Evidence for an association between specific alleles at every locus with B.indicus/B.taurus breed was assessed by using a Logistic Regression model. Significant relationship was discovered only for two loci, TGLA227 (χ2 = 22.30; P < 0.001) and ETH10 (χ2 = 27.70; P < 0.001). It can be assumed that the TGLA227 (77 bp) and ETH10 (209-211 bp) alleles among the SM cattle examined individuals were inherited from a B.indicus ancestor. On the other hand, the TGLA227 (89 bp) and ETH10 (217-219 bp) alleles which prevails among individuals in the LZ group were inherited from a B.taurus ancestor. Thus, the SM cattle presented with high level of taurine/zebuine admixture, which is consistent with the breeding history. Product Description popup.authors Kramarenko Olexander S. popup.nrat_date 2020-04-02 Close