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Information × Registration Number 0219U102423, 0116U004864 , R & D reports Title The application of metabolic engineering approaches for construction of Saccharomyces cerevisiae strains able to overproduction of glycerol under anaerobic conditions popup.stage_title Head Dmytruk Kostiantyn V., Registration Date 28-12-2019 Organization Institute of Cell Biology popup.description2 For the purpose of derepression of the genes involved in glycerol synthesis, vectors containing the GPD1 gene (encoding glycerol-3-phosphate dehydrogenase), GPP2 gene (encoding glycerol-3-phosphate phosphatase) and hybrid ORF GPD1-GPP2fus under the control of a strong constitutive promoter of the ADH1 gene were constructed. These vectors were used to transform the Saccharomyces cerevisiae strain BY4742, as well as the BY/tpi25 strain with reduced triosephosphate isomerase activity. Overexpression of the GPP2 gene did not influence glycerol production in S. cerevisiae, whereas recombinant strains with overexpression of the GPD1 gene or the fusion gene GPD1-GPP2fus produced respectively 8 g/L or 11 g/L of glycerol during alcohol fermentation. The combination of overexpression of the fusion gene GPD1-GPP2fus with a decrease in the activity of the triosephosphate isomerase provided an additional increase in the level of glycerol production - up to 13 g/L, which is 5.6 times more compared to the wild type strain BY4742. Product Description popup.authors Kurylenko Olena O. Semkiv Marta V. Sibirny Andriy A. popup.nrat_date 2020-04-02 Close