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Registration Number

0220U010165, 0118U006867 , R & D reports

Title

Differentiation of various genotypes and plant varieties by estimating the polymorphism of the length of actin introns

Head

Kalafat Liubov O., Кандидат біологічних наук

Registration Date

17-02-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Description

 Bioinformatic search of actin genes in the genomes of Triticum aestivum, Hordeum vulgare and Brassica rapa was performed. It was found that exon regions of actin genes in the studied plant species are highly conserved, while intron regions are hypervariable, which may be useful for the development of DNA markers based on the assessment of intron polymorphism. Design and synthesis of degenerate universal primers was carried out to evaluate the actin gene intron length polymorphism in higher plants. Testing of the developed DNA marker system, which reveals the polymorphism of the intron length of actin genes, was carried out on samples of monocotyledonous and dicotyledonous plants of different taxonomic groups. A DNA marker system evaluating the intron length polymorphism of actin genes allowed genotyping and differentiation of Ae populations qualitatively. Biuncialis in the Crimea, revealing a significant number of polymorphic amplicons of introns of actin genes. High heterogeneity and genetic heterogeneity of the investigated natural populations of Egilopsis have been shown. The analysis of the polymorphism of the length of the introns of the actin genes in the Antarctic species Deschampsia antarctica was carried out. Genetic monomorphism for this type of DNA marker has been established. Genotyping and differentiation of barley (Hordeum vulgare L.), tomato (S. lycopersicum), potato (S. tuberosum) and flax varieties of Ukrainian and Belarusian breeding were also conducted. In general, species-specific DNA profiles with a specific set of fragments of introns of actin genes for all analyzed plant species were obtained. On the whole, DNA markers evaluating the intramine length polymorphism of actin genes made it possible to qualitatively genotype plant species and varieties

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Bilonozhko Yuliya O.
Demkovich Andriy Ye.
Kalafat Liubov O.
Pirko Nadija М.
Pirko Yaroslav V.
Postovoytova Anastasiya S.
Privalikhin Serhiy M.
Rabokon Anastasiia M.