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Information × Registration Number 0220U100249, 0115U005044 , R & D reports Title Production of genetically engineered immunoconjugatesand their application for immunoaffinity chromatography and detection of target antigens popup.stage_title Head Horbаtiuk Oksаnа B., Кандидат біологічних наук Registration Date 14-01-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 Genetically engineered immunoconjugates based on target proteins and marker molecules were characterized. Recombinant fusion protein based on the human interleukin-7 (rhIL7) and bacterial alkaline phosphatase has been designed. It was shown that autoinduction protocol provides a significantly higher level of protein synthesis in E. coli compared with IPTG induction. In vitro method of purification and renaturation of rhIL7-BAPmut from inclusion bodies has been worked out. It has been shown that rhIL7-BAPmut has a specific biological activity after renaturation. The obtained rhIL7-BAPmut protein can be used for screening of immune combinatorial cDNA libraries of immunoglobulins variable genes, that are the sources of specific single chain antibodies. The protein also can be used for qualitative and quantitative analysis of IL-7 receptors. Product Description popup.authors Irodov Dmytro M. Pokholenko Yanina O. popup.nrat_date 2020-04-02 Close