Registration Number
0220U100249,
0115U005044
, R & D reports
Title
Production of genetically engineered immunoconjugatesand their application for immunoaffinity chromatography and detection of target antigens
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Head
Horbаtiuk Oksаnа B., Кандидат біологічних наук
Registration Date
14-01-2020
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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Genetically engineered immunoconjugates based on target proteins and marker molecules were characterized. Recombinant fusion protein based on the human interleukin-7 (rhIL7) and bacterial alkaline phosphatase has been designed. It was shown that autoinduction protocol provides a significantly higher level of protein synthesis in E. coli compared with IPTG induction. In vitro method of purification and renaturation of rhIL7-BAPmut from inclusion bodies has been worked out. It has been shown that rhIL7-BAPmut has a specific biological activity after renaturation. The obtained rhIL7-BAPmut protein can be used for screening of immune combinatorial cDNA libraries of immunoglobulins variable genes, that are the sources of specific single chain antibodies. The protein also can be used for qualitative and quantitative analysis of IL-7 receptors.
Product Description
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Irodov Dmytro M.
Pokholenko Yanina O.
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2020-04-02
