Registration Number
0220U101011,
0117U004234
, R & D reports
Title
Detection of genetic resistance to fusariosis in introgressive lines of wheat with the usage of sensory system on the basis of molecular imprinted polymer membrane
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Head
Ternovskaya Tamara K., Доктор біологічних наук
Registration Date
30-01-2020
Organization
National University of Kyiv-Mohyla Academy
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Object of the study – processes of development of molecular imprinted polymers as nanostructured polymer membranes selective for zearalenone, and optical sensor devices on their basis for determination of resistance to Fusarium in common wheat introgression derivatives.
Aim of the study – development of introgression derivatives T. aestivum / T. miguschovae, and their resistance to Fusarium will be diagnosed on the basis of nanostructured polymer membranes developed in this project using method of molecular imprinting.
Research methods – molecular genetic methods for development and analysis of plant introgression material, computer modeling of nanostructured polymers, synthesis of molecular imprinted polymer (MIP) membranes using method of in situ polymerization, assessment of synthesized MIP membranes for their ability to highly selectively recognize zearalenone.
During the implementation of the project computer modeling of nanostructured polymers capable to selectively recognize zearalenone as marker of wheat Fusarium head blight was conducted. Synthesis of molecular imprinted (MIP) membranes using method of in situ polymerization according to the technology of molecular imprinting was performed. MIP membranes of optimal composition were used as basis for the development of sensor systems for determination of this mycotoxin. For the development of sensor systems which could be effectively used for Fusarium head blight diagnostics outside the laboratory, particularly in field conditions, such an approach was suggested whereas detectors and analyzers of sensor signals modern digital devices – smartphones, were used. Portable sensor systems based on MIP membranes and smartphones were used for determination of zearalenone in both model and real samples, in particular in food samples (flour), and also in developed during the implementation of this project introgression material Triticum aestivum/T. miguschovae.
Product Description
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2020-04-02
