Registration Number
0220U101075,
0119U100134
, R & D reports
Title
To develop a method for detecting of the ASFV DNA on the basis of an isothermal reaction
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Head
Gerilovych Anton P., Доктор ветеринарних наук
Registration Date
31-01-2020
Organization
National Scientific Center Institute of Experimental and Clinical Medicine
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The database containing full genome sequences of African swine fever virus DNA has been created. Genes with unknown functions с717r, b385r, c257l and gene c962r coding putative DNA primase of ASFV has been determined as molecular markers for ASFV DNA detection using LAMP assay. Primers for the chosen fragments has been designed. The primer set amplifying target sequence the most effectively has been used for the futher testing and protocol optimisation. The reaction results can be obtained in 30 min, which is much faster than time to result of conventional PCR and qPCR. Methodology for ASFV DNA detection using LAMP has been developed.
Product Description
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Kyt Maryna Yu.
Lymanska Olga Yu.
Rudova Nataliia H.
Solodiankin Oleksii S.
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2020-04-02
