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Information × Registration Number 0220U101230, 0116U007815 , R & D reports Title Study of biological characteristics of endometrial cancer and determination of endometrioid carcinoma molecular subtypes associated with high malignant potential popup.stage_title Head Бучинська Любов Георгіївна, Registration Date 05-01-2020 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  Based on the analysis of the results of a comprehensive clinical-morphological, IHC and molecular biological study, groups of neoplasms with various phenotypic features both in histological and molecular characteristics were identified. The associations of heterogeneity of the morphological phenotype of EEC with changes in the expression of markers of the epithelial-mesenchymal transition and their relationship with the possible pathways of migration and invasion of tumor cells were determined. It was found that changes in the copy number of c-MYC, HER-2/neu, CCNE1 oncogenes, along with high expression of the corresponding proteins, aneuploid DNA status and low expression level of FOXP3 tumor suppressor in endometrial tumor cells are associated with such indicators of the aggressiveness of the malignant process as high proliferative potential, low degree differentiation and deep myometrial invasion. The aforementioned justifies the prospect of using the studied proteins as components of a multimarker panel to determine the most informative indicators of the aggressiveness of the tumor process in the endometrium. The prognostic significance (the Kullback method and the PanelomiX web method) of the expression of 13 biomolecular markers (p53, FOXP3, p21WAF1/CIP1, E2F1, cyclins E and D1, HER-2/neu, c-Myc, E-cadherin, β-Katenin, vimentin, CD44, CD24) and their optimal combination with expression thresholds was obtained (p53> 45.0%; FOXP3 <14.0%; c-Myc> 10.0%). For the first time, the most informative for specificity and sensitivity (95%) molecular phenotype of EEC - p53highFOXP3lowc-Mychigh – was characterized which allows to identify the highly malignant serous-like subtype of this form of cancer and provides the possibility of an individualized prognosis of the course of the disease for reasoned correction treatment of patients. Product Description popup.authors Brieieva Olga V. Buchinskaya Lubov G. Glushchenko Nadiia M. Kashuba Elena V. Nesina Iryna P. Iurchenko Nataliia P. popup.nrat_date 2020-04-02 Close