Registration Number
0220U101254,
0117U000663
, R & D reports
Title
Experimental morfofunctional studies of changes of barrier functions of skin at action of nanoparticles of heavy metals and mechanism of their resorption through the uninjured skin
popup.stage_title
Head
Chernyuk Volodymyr I., Доктор медичних наук
Registration Date
07-02-2020
Organization
State Institution "Kundiiev Institute of Occupational Health of National Academy of Medical Sciences of Ukraine"
popup.description2
Object of the study: morpho-functional changes of barrier skin function under long-term (1month) effect of nanoparticles (NPs) colloids of metal compounds on the uninjured skin of rats (Wistar): PbS, of 12,5 nm and 100 nm size; Fe2O3, 19 nm and 75 nm size; Ag0, AgInS2 and CuInS2/ZnS of 15-20 nm size, standardized by the weight part of a metal (0,01 М). Subject of the study: morpho-functional changes of epidermis, dermo-epidermic junctions, dermis and appendages of the skin. Purpose of the study: experimental study of morpho-functional damage of skin barrier properties in rats under a long-term intervention of compounds of heavy metal NPs (Pb, Cu, Ag та Fe) and determination of mechanism of their resorption through the skin. Study design: toxicological, histological, histochemical, submicroscopic, luminescent and polarization microscopy, as well as morphometry and variation statistics methods. Results and their novelty: It is stated for the first time, that long-term effect of metal NP compounds on the uninjured skin leads to morphological changes, which are seen at microscopic and submicroscopic levels in all skin layers of epidermis, dermis and appendages. All changes have signs of destruction and point to the skin barrier function damages. Submicroscopic changes of membrane and cytoplasm of corneocytes of the stratum corneum and keratinocytes of all layers, as well as changes of the basal membrane structures and hair follicles and holocrine glands determine main ways of metal NP resorption through the uninjured skin of rats under their long-term intervention. Mechanisms of intracellular, transcellular and intrafollicular transport play a significant part. The results prove that not only toxic properties are of great importance in providing mechanisms of resorption through the skin of metal NPs, different by their physico-chemical parameters, but, also, their size, type, concentration and time of their application onto the skin.
Product Description
popup.authors
popup.nrat_date
2020-04-02
