Registration Number
0220U101477,
0115U003683
, R & D reports
Title
Development of nanobiomedical technology for recording of cell electrical activity by means of quantal dots.
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Head
Bilan Pavlo V., Доктор біологічних наук
Registration Date
11-02-2020
Organization
Bogomolets Institute of Physiology
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This research paper proposes a new method for recording the electrical network activity of excitatory cells of an organism using semiconductor quantum dots (QD). This task requires the simultaneous measurement of electrophysiological and fluorescence signals from live functioning cells with high temporal resolution. In the course of the project, we have jointly tested our electrophysiological and digital fluorescence microscopy equipment to evaluate the temporal resolution of the proposed method. For this purpose, we expressed in neurons fluorescence proteins that change the fluorescence in the plasma membrane depending on the level of the membrane potential and have developed software that can visualize these changes, which is similar to the calculated changes in the fluorescence of QD. We have also refined the use of potential and current fixation techniques to induce controlled changes in the membrane potential of neurons. The involvement of the first method allowed us to investigate the dependence of fluorescence of fluorescence proteins in the plasma membrane of neurons on the level of membrane potential and to quantify their concentration in living functioning neurons of the hippocampus. Our further studies have shown that commercially available quantum dots can be embedded in the plasma membrane of living normally functioning neurons and remain there for the time necessary to carry out membrane potential measurements. The analysis confirms that the potential dependence and brightness of commercially available quantum dots allows for fluorescence registration of the electrical activity of living normally functioning cells. The new quantum dot biosensor nanosystem, in combination with fluorescence microscopy equipment based on high-resolution fast CCDs, or confocal and multi-quantum microscopes, can be used for quantitative, fast, and spatially resolving measurements of potential cells.
Product Description
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Ivanova Svitlana Yu.
Bilan Pavlo V.
Voitenko Nana V.
Dovgan Olexandr V.
Dromaretsky Andriy V.
Kononenko Mykola I.
Korogod Sergiy М.
Palyvoda Ludmila G.
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2020-04-02
