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Information × Registration Number 0220U101532, 0117U004276 , R & D reports Title To investigate the molecular-cell interaction between mesenchymal and neuronal stem cells with the fibrin matrix components in vitro to activate the neuroregenerative processes popup.stage_title Head Tsymbaliuk Vitalij I., Доктор медичних наук Registration Date 12-02-2020 Organization The State institution "Romodanov neurosurgery institute, National academy of medical sciences of Ukraine" popup.description2 Nerve cell culture experiments using 3D STP (platelet-enriched fibrin) matrix have demonstrated that it is a favorable environment for them, lacking cytotoxic action. Within 2 weeks of cultivation around and within the fibrin matrix, growth zones are formed, densely populated by cells, which form a network of processes and establish contact. In highly specialized neurons n. raphe conserves serotonin, and the marker genes of the precursors of serotoninogenesis Pet1 and Nkx 2.2 are expressed. Wharton bone marrow MSCs and bone marrow cultured in the 3D STP matrix also demonstrated high viability, presence of CD105 + cells, formation of a confluence zone within (10-14) days, depending on the type of MSCs and ascending cell count. It has been established that the TFT matrix is ​​a carrier of positional information for stem and progenitor cells that can be used for their directional differentiation. Cultivation of bone marrow MSCs in the matrix with incorporated neurogenically active substances: RA (retinoic acid), BDNF (brain-derived neurotrophic factor) and NGF (nerve growth factor) stimulate in them the expression of marker neurogenic genes Nes, Gfap, Olig3 and Olig2 on the morphological phenotype of MSCs, making it similar to different cells of the nervous system. Thus, the 3D STP matrix is ​​a universal substrate for the maintenance of vital activity and functional activity of cells of different origin ex vivo, has great potential to stimulate their differentiation in a given direction. Product Description popup.authors Vasilyeva Irina G. Verbovska Svitlana A. Halanta Olena S. Dmitrenko Angela B. Makarova Tatyana A. Oleksenko Natalia P. Snitchar Natalia D. Tsubko Olga I. Chopyk Nataliia H. Shuba Irina M. popup.nrat_date 2020-04-02 Close