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Registration Number

0220U101654, 0118U006868 , R & D reports

Title

"Green synthesis" of silver-containing nanoparticles and the development of methods for their practical use

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Head

Pirko Yaroslav V., Кандидат біологічних наук

Registration Date

17-02-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

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 According to the results of a literature search on the mycosynthesis of silver-containing nanoparticles, 7 species of the genus Pleurotus were selected for experiments: Pleurotus calyptratus, P. djamor, P. dryinus, P. eryngii, P. ostreatus, P. pulmonarius, P. salignus. The antibacterial activity of synthesized P. eryngii silver-containing nanoparticles against standard pathogenic bacteria was investigated: Staphylococcus aureus UMC B 4001 (ATCC 65388), Bacillus subtilis UMC B-901 (ATCC 6633), and Escherichia coli6 (UMCB9). A weak antibacterial effect of mycelial filtrate and culture fluid P. eryngii against B. subtilis and significant growth inhibition of S. aureus mycelial filtrate were revealed. Under optimal conditions (temperature 26 ± 2 ° C, pH 5.0-5.5), the ability of representatives of the genus Pleurotus to synthesize biomass in glucose-peptone-yeast and potato-dextrose liquid media was studied. Biomass growth in different media is found to occur at almost the same level and is highest on the 14th day of cultivation. The silver-containing nanoparticles synthesized using the “mushroom matrix”, have a rounded shape and their size is 5-20 nm, and are characterized by weak antimicrobial activity. Solutions with silver-containing nanoparticles were absorbed at a wavelength of 430 nm and had a relatively low fluorescence emission within 600 nm. The 3D fluorescence spectra showed enhanced fluorescence at λex≈450 nm and λem≈600 nm in the presence of intact (all samples) and fibrillar insulin (P. eringii), compared to fluorescence in the buffer. That is, the nanoparticles formed using P. eringii had selectivity for insulin fibrils compared to intact protein. Modification of cotton fabric with silver-containing nanoparticles increases the absorption of UV-B in the range of 79.3% to 87.0%, and for UV-A in the range of 96.4% to 97.8%.

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Yemets Alla I.
Isayenkov Stanislav V.
Bilonozhko Yuliya O.
Barshtejn Viktor Yu.
Borova Mariya M.
Buziashvili Anastasiya Yu.
Horiunova Inna I.
Danylenko Ihor A.
Demkovich Andriy Ye.
Kalafat Liubov O.
Kvasko Anna Yu.
Korhovyy Vitaliy I.
Krasnoporova Elena Ye.
Krupodorova Tetiana A.
Malienko Vadim A.
Nesterenko Yevheniya О.
Pirko Nadija М.
Pirko Yaroslav V.
Privalikhin Serhiy M.
Rabokon Anastasiia M.
Ratushniak Volodymyr V.
Smertenko Petro S.

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2020-04-02