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Registration Number

0220U101946, 0119U102575 , R & D reports

Title

New approaches to obtaining and assessing the quality of genetically safe water

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Head

Bolgova Olena S., Кандидат технічних наук

Registration Date

28-02-2020

Organization

A.V Dumansky Institute of Colloid Chemistry and Water Chemistry

Description

 The main purpose of scientific research is to develop new approaches to the assessment and production of genetically safe drinking water using physiologically active substances that affect toxic substances and pathogens. No work has been found on the use of anthocyanins in the water treatment process. Therefore, we first decided to evaluate the effectiveness of the use of grape anthocyanins in water treatment by examining their toxic effects by the most informative and simple method in the modern world - biotesting. Based on the data obtained, it was found that water samples with anthocyanins at concentrations of 0.02 and 0.05 g / l had no acute and chronic toxic effects on the organisms of the aquatic organisms (fish, hydra and teriodophagus). Also, these concentrations did not cause nuclear disturbances in fish blood cells and showed no cytotoxic effect (0 ‰; 0.33 ‰, which corresponds to norms). Water samples with anthocyanin concentrations of 0.02 and 0.05 g / l according to the total effect of size and weight characteristics of onion roots were + 8.04% and + 1.73%, respectively, which classifies them as safe water. According to the results of complex biotesting, it can be concluded that water samples with these concentrations meet the standards of DSTU 7525: 2014 “Drinking water. Requirements and methods of quality control ». Indicators for biotesting of water samples with anthocyanin concentration of 0.14 g / l meet the requirements of normative documents for general toxicity, but do not meet the requirements for cytotoxicity (cytotoxic effect 0.66 ‰ at the rate of 0.33 ‰). Water samples with anthocyanins at concentrations of 0.17; 0.23, 0.33, and 0.4 g / l showed both acute and chronic toxicity, as well as cytotoxicity not in accordance with DSTU 7525: 2014.

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Holovkov Andrii M.
Pelishenko Oleksandra V.