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Information × Registration Number 0220U102457, 0114U006297 , R & D reports Title The pathological role of polymorphism of single nucleotides is in the development of the most widespread pathological processes and diseases of human popup.stage_title Head Ataman Olexandr V, Доктор медичних наук Registration Date 13-03-2020 Organization Sumy State University popup.description2  The vascular diseases of the brain are one of the most important and complex problems of clinical medicine, due to their significant prevalence, high disability and mortality. In the structure of vascular diseases of the brain, the ischemic stroke itself is one of the most common clinical forms of acute disorder of cerebral circulation. In the pathogenesis of ischemic lesion of the brain the disturbance of endothelium function plays an important role. The function of endothelin-1 and endothelin receptor of type A is definitely determined by the structural features of their genes. Therefore, the role of single nucleotide polymorphisms of EDN1 and EDNRA genes in the development of ischemic atherothrombotic stroke is of great importance. The main method for genotyping was the polymerase chain reaction followed by the analysis of the restriction fragment length polymorphism (PCR-RFLP). The determined rates of minor alleles for polymorphic variants of the EDN1 and EDNRA genes in persons without ischemic stroke are in accordance with most European populations and are 0.2 for Lys198Asn-polymorphism and 0.46 for the C+70G polymorph site. There is a significant difference in the distribution of allelic variants between the group of patients with IAS and the individuals in the control group for Lys198Asn of the EDN1 gene (P = 0.008), but the relationship between variants of genotypes in the comparison groups on C+70G-polymorphism of the EDNRA gene does not differ substantially (Р = 0.426) Product Description popup.authors Harbuzova Victoriia Yu. Matlay Olga I Oleshko Tetiana B Smirnov Oleh Yu Starchenko Anton Yu popup.nrat_date 2020-04-01 Close