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Information × Registration Number 0220U102586, 0119U000577 , R & D reports Title Dose phenotypes, functionalities and molecular genetic characteristics of chubby clitoris due to authorities of the stanburia and obruntuvaty popup.stage_title Head Khranovska Natalia M, Registration Date 07-04-2020 Organization National Cancer Institute popup.description2 Methodical approaches to three-dimensional spheroidal growth of human and mouse tumor cells in vitro have been developed to enrich cultures with cells with stem properties. It was established that on the 6th day of cultivation of the CFL adhesive culture with the addition of 0.2% carboxymethylcellulose, the largest number of tumor spheroids (PS) with the maximum sizes was formed. According to the molecular genetic profile, the additional use of 0.2% carboxymethylcellulose did not contribute to the generation of a significant number of cells in the CFL PS bearing stem cell markers. It was found that PS of non-small cell lung cancer MOR on the 6th day of cultivation with the addition of 0.2% carboxymethylcellulose form a side population (SP), characterized by an increase in the exclusion index of dye R-123 compared with adhesive PCs (p? 0.05). It was found that obtained on the 6th day of cultivation of PS cells of MOR line by their phenotypic characteristics (CD90 and CD117) do not differ from cells of monolayer MOR. It was found that the mRNA expression levels of CD44 and CD133 surface markers in MOR PSs were significantly higher than those in the monolayer of adhesive PCs (p? 0.05). It was found that mRNA expression levels of all investigated stem cell markers (ALDH1, SOX2, Nanog, CD44, and CD133) were significantly lower in the supernatant cells compared to the values ??in the monolayer of the adhesive cells and PS. Obtained PS MOR in the conditions of 0.2% carboxymethylcellulose in the absence of embryonic calf serum (ETS) have some properties of stem cells, however, the amount of PSC is still insufficient to use them as a source of tumor antigens in the design of DC vaccines, so in the following studies EGF cultivation conditions and growth factor.  Product Description popup.authors Horbach Oleksandr I. Skachkova Oksana V Khranovska Natalia M popup.nrat_date 2020-07-03 Close