Registration Number
0220U104122,
0118U001828
, R & D reports
Title
Determination of essential parameters and development of a new sensing methodology for assessment of toxicity of aerosol particles and analysis of the neurotoxic action of mercury and persistent organic pollutants on the basis of experimental data on the effect on the functioning of nerve cells and the strategy of neuroprotection.
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Head
Borysova Tetiana O., Доктор біологічних наук
Registration Date
10-12-2020
Organization
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine
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The preparation of aerosol nanoparticles obtained by combustion of organic substances has been developed and characterized, which allows to evaluate the mechanism of aerosol toxicity and is extremely relevant for the development of methodology for its monitoring and sensing in the environment, as well as ways of neutralizing toxic effects. In particular, the preparations of aerosol particles obtained by combustion of wood and plastic were created. The particle size distribution was analyzed and the presence of nanoscale particles in smoke aerosol preparations from wood and plastic combustion was shown. The mechanism of toxic effects of particles suspended in water of the preparation of smoke aerosol from the combustion of plastic, namely, a significant decrease in the transporter-mediated accumulation of excitatory neurotransmitter glutamate and inhibitory neurotransmitter GABA in rat brain nerve terminals. It is shown that particles of the preparation of smoke aerosol from the combustion of plastic can lead to an imbalance of excitation-inhibition processes in the central nervous system. It is shown that H2O2-stimulated release of L-[14C]glutamate and [3H]GABA from the rat brain nerve terminals does not change in the presence of smoke components from plastic combustion. The effect of size, shape and structure of micro- and nanosized particles on their toxicity was analyzed. The identify mechanisms for the development of neurotoxicity by the action of mercury, complicated by the presence of biomass combustion particles. The neurotoxic effect of mercury is due to an increase in the extracellular level of the excitatory neurotransmitter, glutamate. The determining the mechanisms of neurotoxicity by the complex action of mercury, complicated by the presence of particles from biomass combustion, showed that the effect of mercury on extracellular glutamate level in nerve terminals changes in the presence of nanoparticles.
Product Description
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Borisov Arseniy A
Dudarenko Marina V
Krysanova Nataliya V
Paliienko Konstantin O
Pastukhov Artem O
Pozdnyakova Natalia G
Tarasenko Alla S
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2020-12-10
