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Information × Registration Number 0220U104197, 0120U104114 , R & D reports Title Creation of yeast producers of new flavin antibiotics popup.stage_title Head Dmytruk Konstantyn V., Доктор біологічних наук Registration Date 14-12-2020 Organization Institute of Cell Biology popup.description2  A selective marker, the SAT gene, which confers resistance to the antibiotic norseotricin, was used to develop a multicopy integration system for C. famata yeast. A collection of yeast transformants at increasing concentrations of antibiotic (40, 75, 100, 150 mg / l) in the medium was obtained. To develop the CRISPR-Cas9 system, a series of recombinant plasmids containing the CAS9 gene under the control of the strong constitutive promoter of the C. famata TEF1 gene, the homologous promoter of the MAL2 gene, and the heterologous constitutive promoter of the ENO1 gene Candida albicans were constructed. The expression of the directing RNA sequence (gRNA) was performed under the control of the TEF1 promoter, and the gRNA sequence was flanked by the alanine tRNA site from the 5'-end and the HDV virus ribozyme sequence from the 3'-end. for expression in the genome of C. famata The corresponding synthetic constructs were obtained FMN1 genes C. famata, rosB and rosA S. davaonensis were cloned under the control of the TEF1 gene promoter in integrative plasmids The IMH3 gene Debaryomyces hansenii was cloned into the corresponding plasmids. This gene provides resistance to mycophenolic acid and will be used as a selective marker for the selection of target yeast transformants. Key words: genetic engineering, yeast, Candida famata, roseoflavin, aminoriboflavin CRISPR-Cas9, Streptomyces davaonensis, rosB genes, rosA Product Description popup.authors Andrejeva Julia A. Dmytruk Olena V. Petrovska Yana O. Sibirny Andrij A Faiura Liubov R. Fedorovych Dariya V. popup.nrat_date 2020-12-14 Close