Registration Number
0220U104270,
0120U105264
, R & D reports
Title
The influence of antiviral and thrombolytic drugs on the model membranes of viral particles and eukaryotic cells.
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Head
Borysova Tetiana O., Доктор біологічних наук
Registration Date
15-12-2020
Organization
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine
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Processing of literature data on viral membranes, in particular SARS-CoV-2 virus membranes, setting up instruments and purchasing reagents for experiments, setting up project administration and coordination processes, development in vitro model membranes and determining the influence of surface curvature on lipid ordering. The properties of the monolayer of vesicles do not depend on their composition, but depend on the curvature of the surface of each of the monolayers, which can be used to reduce the activity of mechanically sensitive membrane proteins. Such regulation of membrane properties occurs when a virus (an object with a large surface curvature) interacts with the plasma membrane of cells (an object with, in fact, a flat surface), and the nature of such interaction will be affected by amphiphilic drugs that can potentially change patterns of behavior. membrane receptors, transporters, etc. In order to adjust the devices on the example of a model neuroactive substance, it was shown that the electrophysiological method "planar bilayer lipid membrane" was 100 times more sensitive to the registration of membrane-active action and nonspecific violations of lipid bilayer integrity of the model substance compared to radioisotope method. The developed integrated approach will be used to analyze the effect of membranotropic antiviral drugs on the integrity of the lipid bilayer.
Product Description
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Krysanova Nataliya V
Panas Ihor D
Pastukhov Artem O.
Pyrshev Kyrylo O.
Pozdnyakova Natalia H.
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2020-12-15
