Registration Number
0220U104375,
0116U000416
, R & D reports
Title
To investigate the levels of molecular-genetic polymorphism of plant collections for species of the genus Miscantus as well as the genus Salix
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Head
Royik Mykola V, Доктор сільськогосподарських наук
Registration Date
16-12-2020
Organization
Institute of Bioenergy Crops and Sugar Beet of National Academy of Agrarian Sciences of Ukraine
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The spectra of amplicons obtained using RAPD, ISSR and SSR primers allow to differentiate representatives of the genus Miscanthus of different species, according to the established difference in the number of loci for each representative of the species. The expediency of using primers P 816 and RAPD 2 for differentiation of different groups of genotypes of M. sacchariflorus, M. gigantheus, M. sinensis species has been established. Therefore, it can be argued that the use of these primers for breeding practice allows to assess the genetic diversity of existing species of the genus Miscanthus.
RAPD 2, ISSR, and microsatellite primers Sa 54B, SB 38, and SB 85 were used to evaluate the molecular genetic polymorphism of the Salix collection. Primer ISSR 4 detected 570- and 720-bp amplicons. with a frequency of 0.2 and can be used to differentiate members of the genus Salix. . This primer can be used to differentiate members of the genus Salix
Product Description
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Babiazh Anna I
Hryhorenko Nataliia
Royik Mykola V.
Cherednychok Oksana I
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2020-12-16
