Звіт по науково-дослідній роботі. Колибо Денис Володимирович. Розробка нових технологій створення біочутливих функціональних матеріалів для застосування у діагностичних системах медико-біологічного призначення. Розділ 2. Імунохімічна характеристика різних рекомбінантних білків, перспективних для використання у діагностичних системах медико-біологічного призначення.

Registration Number

0220U104540, 0118U002331 , R & D reports

Title

Development of new technologies for the creation of biologically sensitive functional materials for use in diagnostic systems for medical and biological purposes. Stage 2. Immunochemical characteristic of various recombinant proteins, promising for use in diagnostic systems for medical and biological purposes.

popup.stage_title

Head

Kolyba Denys V., Доктор біологічних наук

Registration Date

22-12-2020

Organization

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

popup.description2

 Recombinant proteins (B-subunit of diphtheria toxin, human sHB-EGF growth factor, Chlamydia trachomatis heat shock protein – HSP-60) with various polypeptide tags, as well as monoclonal antibodies against prostate-specific antigen (PSA) were obtained. The sandwich-IEA test-system based on immobilization of antibodies on ammonium perchlorate-functionalized polystyrene surface for quantification of free PSA has been developed. A comparative analysis of the effects of polystyrene chemical modification with ammonium perchlorate on a sorption of the recombinant HSP-60 proteins with different tags was carried out. It was shown that the most effective method used the functionalization of polystyrene surface with ammonium perchlorate (relative degree of hydrophilization 4.8) and recombinant proteins rHSP-60-GST or rHSP-60-His6 (at concentration of 5 μg/ml or more), that allowed to increase the IEA signal by about 1.5 times and positivity index by 30% without reducing of nonspecific interaction level. The developed technique of polystyrene surface functionalization can be used in diagnostic systems for biomedical purposes. The developed test-system for quantification of free PSA can be used to diagnose and monitor the effectiveness of prostate cancer therapy. The IEA technique for detecting IgG-specific antibodies to HSP-60 can be used in development of the test-system for urogenital chlamydia diagnosis.

Product Description

popup.authors

Galkin Oleksandr Yu.
Katashinskaya Daryna O.
Komisarenko Sergij V.
Krynina Olha I.
Kulyk Yaroslava S.
Labintsev Andriy Yu.
Romanyuk Svitlana I.
Siromolot Andriy A.

popup.nrat_date

2020-12-22