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Information × Registration Number 0220U104692, 0120U100197 , R & D reports Title Target gene delivery into mammalian cells using novel native biocompatible polymeric nanomaterials popup.stage_title Head Finiuk Nataliya S., Кандидат біологічних наук Registration Date 24-12-2020 Organization Institute of Cell Biology popup.description2  It was investigated 14 novel polymeric carriers based on poly (2-dimethylamino)ethyl methacrylate (polyDMAEMA) as carriers for gene delivery in mammalian cells. It was detected effective immobilization of plasmid DNA pEGFPc-1 with polymeric carriers based on polyDMAEMA: mPEG475DM1, F8DM4, F8DM2, F8DM1, F16DM2, F16DM1, F12DM2, F12DM1, BA-DM1-1-1 , LA-DM2, LA-DM1. Different buffer systems and solutions were used (saline, Ringer's solution, Hanks' solution, phosphate-buffered saline (PBS), Tris-saline buffer (TBS), serum-free culture medium (DMEM or RPMI1640 (depending on studied cell line) were used for polymer / plasmid DNA complexation and subsequent transfection of the cells. The highest efficiency of transfection of mammalian cells was observed when using the polymer/plasmid DNA complex formed in serum-free culture medium compared to the application of complexes formed in other buffers. РolyDMAEMA were tested as carriers to deliver plasmid DNA pEGFPc-1 into mammalian cells (HEK293 and HEK293T of human embryonic kidney, MCF-7 human breast adenocarcinoma, HeLa cervical carcinoma, H CT116 human colorectal carcinoma, U251MG human glioblastoma, human HepG2 hepatocarcinoma, A549 human lung carcinoma) in vitro with a transfection efficiency of 1.0-67.9%. PolyDMAEMA carriers at the working concentration of 0.0002% showed low toxicity towards mammalian cells (94.8-79.9% of living cells were observed), while under the action of polyethyleneimine - 60.9-53.0% of living cells were detected. The studied polyDMAEMA carriers caused lower amount of single-strand breaks in the DNA molecule of HEK293 cells: the rate of DNA damage Olive tail moment was 2.7-3.6%, compared with polyethyleneimine (Olive tail moment was 5.8%). The polymers do not possessed genotoxic and mutagenic activity (they were not able to induce gene mutations in the Ames test, and did not increase the frequency of chromosomal aberrations and micronuclei in the bioassay using Allium cepa). Product Description popup.authors Kozak Yuliya S. Finiuk Nataliya S. popup.nrat_date 2020-12-24 Close