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Information × Registration Number 0220U104721, 0119U001967 , R & D reports Title Development of highly sensitive enzyme-linked immunosorbent assay systems for the diagnosis of Epstein-Barr virus popup.stage_title Head Tukаlo Mykhаilo A., Доктор біологічних наук Registration Date 26-12-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  Technological regulations for the expression of recombinant proteins of Epstein-Barr virus antigens - capsid of BFRF3 virus (p18) and BLRF2 (p23) and nuclear antigen of EBNA1 protein virus (p79) in bacterial producer cells and their purification in preparative quantities were developed. The validation of the procedure for conducting optimal variants of enzyme-linked immunosorbent assay using standard ELISA equipment, thermoshaker and automatic analyzer was developed and validated. The stability of test systems during storage is determined. A comparative evaluation of the diagnostic characteristics of test systems with their commercial counterparts from leading manufacturers. Technological regulations for the manufacture and instructions for the use of three test systems for the diagnosis of Epstein-Barr virus by enzyme-linked immunosorbent assay: IgG to VCA antigens of Epstein-Barr virus; IgM to VCA Epstein-Barr virus antigen; IgG to Epstein-Barr virus nuclear antigen. Product Description popup.authors Garifulin Oleg M. Gudzera Ol'ga Yo. Kovalenko Oksana P. Kryklyvyi Ivan A. Krups'ka Iryna V. Savins'ka Liliya O. Filonenko Valeriy V. Khoruzenko Antonina I. popup.nrat_date 2020-12-26 Close