Registration Number
0220U104721,
0119U001967
, R & D reports
Title
Development of highly sensitive enzyme-linked immunosorbent assay systems for the diagnosis of Epstein-Barr virus
popup.stage_title
Head
Tukаlo Mykhаilo A., Доктор біологічних наук
Registration Date
26-12-2020
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
popup.description2
Technological regulations for the expression of recombinant proteins of Epstein-Barr virus antigens - capsid of BFRF3 virus (p18) and BLRF2 (p23) and nuclear antigen of EBNA1 protein virus (p79) in bacterial producer cells and their purification in preparative quantities were developed.
The validation of the procedure for conducting optimal variants of enzyme-linked immunosorbent assay using standard ELISA equipment, thermoshaker and automatic analyzer was developed and validated.
The stability of test systems during storage is determined.
A comparative evaluation of the diagnostic characteristics of test systems with their commercial counterparts from leading manufacturers.
Technological regulations for the manufacture and instructions for the use of three test systems for the diagnosis of Epstein-Barr virus by enzyme-linked immunosorbent assay: IgG to VCA antigens of Epstein-Barr virus; IgM to VCA Epstein-Barr virus antigen; IgG to Epstein-Barr virus nuclear antigen.
Product Description
popup.authors
Garifulin Oleg M.
Gudzera Ol'ga Yo.
Kovalenko Oksana P.
Kryklyvyi Ivan A.
Krups'ka Iryna V.
Savins'ka Liliya O.
Filonenko Valeriy V.
Khoruzenko Antonina I.
popup.nrat_date
2020-12-26
