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Information × Registration Number 0221U100371, 0120U100747 , R & D reports Title Study of molecular-genetic features of pathogenesis of Ph-negative myeloproliferative neoplasia in persons who have been exposed to ionizing radiation due to the Chernobyl accident popup.stage_title Head Klymenko Sergyi V., Доктор медичних наук Registration Date 05-01-2021 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2 Object of study: genomic DNA of 330 patients with Ph-negative myeloproliferative neoplasia (MPN), who were exposed to ionizing radiation (IR) as a result of the Chernobyl accident. The aim of the work is to study the spectrum of genomic changes in groups of patients with Ph-negative MPNs in specific genes - drivers (major mutations) with subsequent identification of other potential genes that may be involved in the development of tumors. Research methods - genomic DNA was obtained from peripheral blood leukocytes, identification of major mutations was performed by polymerase chain reaction, additional genetic variants were studied by complete exomic and target sequencing, performed statistical processing of the obtained data. It was confirmed that patients with a history of radiation are more likely to have a negative status for major driver mutations in the genes JAK2, MPL and CALR than those who became ill spontaneously (25.7% vs. 15.7%, p = 0.046). The mean number of additional mutations in patients with radiation-associated primary myelofibrosis (PMF) was 4.8 per case, while spontaneous - 3.1 (p = 0.024). In the group of patients with PMF with a radiation history and triple-negative mutation status, the most frequently altered genes were EZH2, DNMT3A, TET2 and ATM. Mutations in the DTA genes (10 of 25 to 4 of 33 cases, respectively, p = 0.03) were detected much more often in patients with MPN with a history of radiation than in patients with spontaneous disease, which indicates the peculiarities of radiation pathogenesis and possibly more frequent development precedence. diseases of clonal hematopoiesis of unclear potential.   Product Description popup.authors Klimuk Bogdana T NEUMERZHYTSKA LIUBOV V Poluben Larysa O. popup.nrat_date 2021-01-05 Close