Registration Number
0221U100414,
0120U100748
, R & D reports
Title
Investigation of the possibility of modification by astaxanthin of radiation-induced in vitro genome injuries in peripheral blood lymphocytes of individuals with BRCA1 gene mutations.
popup.stage_title
Head
Klymenko Sergyi V, Доктор медичних наук
Kurinnyi Denys A., Кандидат біологічних наук
Registration Date
05-01-2021
Organization
State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine"
popup.description2
The object of the study is peripheral blood lymphocytes of conventionally healthy individuals and individuals with mutations in the BRCA1 gene. The aim of this work is to study the effect of a mutation in the BRCA1 gene on the development of genomic instability in human peripheral blood lymphocytes. Research methods: molecular genetic - the method of polymerase chain reaction and electrophoresis for the separation of the amplification PCR - search for mutations 5382insC, 185delAG, 300T> G, 4153delA in the BRCA1 gene; Comet assay method.
Individuals carriers of the 5382insC mutation in the BRCA1 gene have been identified. A comparison of the level of genomic instability in peripheral blood lymphocytes of conventionally healthy individuals and persons carrying a mutation in the BRCA1 gene has been carried out.The features of the development of genomic instability in persons carrying the 5382insC mutation in the BRCA1 gene have been revealed - the presence of a cell pool with a high level of DNA damage on the background of the absence of an increase in the level of chromosomal aberrations and activation of the checkpoint - control at the S stage of the mitotic cycle. Apoptosis is the key mechanism for regulating the development of genomic instability in lymphocyte cultures of individuals who are asymptomatic carriers of the 5382insC mutation in the BRCA1 gene.
Product Description
popup.authors
Verbylenko Roksana M.
Demchenko Olena M.
Mehkeda Ludmila V.
Rybchenko Lyudmila A.
Stefanovych Hanna V.
popup.nrat_date
2021-01-05
