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Information × Registration Number 0221U100522, 0120U103405 , R & D reports Title Recombinant strains of the yeast Komagataella phaffii producing flavin antibiotics aminoriboflavin and roseoflavin popup.stage_title Head Sibirny Andrij A., Доктор біологічних наук Registration Date 07-01-2021 Organization Institute of Cell Biology popup.description2 During the previous stage of work, recombinant strains of yeast K. phaffii were constructed, containing expression cassettes prTEF1-FMN1-prGAP-rosB and Y33-FMN1-rosB-rosA, which were able to synthesize fluid in addition to riboflavin and other fluorescing compounds. At this stage of research, a simple and effective method of purification of aminoriboflavin has been developed, its preparative amounts have been obtained and it has been shown that it has a marked inhibitory effect on some strains of Listeria monocytogenes ATCC 1913, S. aureus ATCC 25923 and methicillin resistant strain S. aureus ( MRSA). Transformants containing the Y33-FMN1-rosB-rosA cassette were found to produce pink flavin, which was identified as roseoflavin by spectral analysis (λmax = 505 nm), high performance liquid chromatography, and mass spectrometry. The conditions for culturing these transformants in a fermenter were optimized, a quantitative analysis of the content of various flavins was performed, a simple and effective method of purification of roseoflavin was developed and it was obtained in preparative quantities. In the flasks, the constructed strain prTEF1-FMN1-prGAP1-rosB-prGAP1-rosA produced 1.2 mg / l of roseoflavin. Under cultured conditions in a 1 L laboratory fermenter, the strain produced 46 mg / L of roseoflavin. Key words: GENE ENGINEERING, YEAST, KOMAGATAELLA PHAFFII, AMINORIBOFLAVIN, ROSEOFLAVIN, FLAVINMONONUCLEOTIDE Product Description popup.authors Dmytruk Kostiantyn V. Fayura Lubov R Fedorovych Dariia V Tsyrulnyk Andrii O. popup.nrat_date 2021-03-17 Close