Registration Number
0221U100685,
0116U001055
, R & D reports
Title
Genetic Value of Sunflower Forms - Carriers of Genes of Resistance to the Most Virulent Downy Mildew Races for Effective Crop Breeding
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Head
Zviahintseva Anna M., Кандидат сільськогосподарських наук
Registration Date
08-01-2021
Organization
Plant Production Institute nd. a. V. Ya. Yuriev
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Study object - genes of resistance of parental sunflower lines to the most aggressive races of the downy mildew pathogen. Purpose - to find genetically valuable forms of sunflower with target genes of resistance to the downy mildew pathogen (Plasmopara helianthi Novot. F. helianthi) using DNA markers. Methods - phytopathological, molecular-genetic, statistical. In accordance with the research programs for the period of 2016-2020: Fifteen resistant sunflower accessions were analyzed by PCR using nine STS markers and three SSR markers. We identified Pl5, Pl6, Pl8 loci in them and distinguished 6 sunflower genotypes with combined genes of resistance to the downy mildew pathogen, which were submitted for testing to the Laboratory of Sunflower Genetics and Breeding of the PPI nd.a. VYa Yuriev NAAS; Methodical recommendations "Marker-Assisted Breeding for Resistance to Major Sunflower Diseases" and "Catalog of Genetic Value of Inbred Sunflower Lines and Winter Bread Wheat Varieties" were implemented into the research and educational processed of KhNAU nd.a. VV Dokuchaiev; Thirteen valuable sunflower accessions carrying the PL6 gene of resistance to the downy mildew pathogen, which had been identified by STS markers (НаР2, НаР3), were introduced into research of the Laboratory of Sunflower Genetics and Breeding of the PPI nd.a. VYa Yuriev NAAS; Monograph "Methodological Principles of Sunflower Breeding for Resistance to Major Diseases" is used in the research and educational processes of KhNAU nd.a. VV Dokuchaiev. The study was conducted with methodological approaches used in international practice. Thus, the laboratory experiments revealed sunflower lines with varying in their responses to the downy mildew pathogen. In addition, genotypes - carriers of different genes of resistance to the downy mildew pathogen (Pl. helianthi) were crossed, and the presence of these genes was established by hybridological analysis
Product Description
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Bilynska Olena V.
Kucherenko Yehor Yu.
Lutsenko Tetiana M.
Makliak Kateryna M.
Syvenko Valentyna I.
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2021-03-17
