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Registration Number

0221U100883, 0118U000133 , R & D reports

Title

Develop complex system of monitoring and eradication associated economically important viral infections of cattle, harmonized to EU.

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Head

Kornieikov Oleksаndr M., Кандидат ветеринарних наук

Registration Date

11-01-2021

Organization

National Scientific Center Institute of Experimental and Clinical Medicine

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 According to the results of epizootological monitoring of cattle in 18 farms from 10 regions, it was determined that animal diseases for viral pneumoenteritis of cattle were registered in all regions where studies were conducted and in 100% of cases were complicated by pathogens of bacterial etiology. In the etiological structure of bovine pneumoenteritis, complex viral associations (IBR and VD; IBR and PI-3; VD and PI-3; IBR and RSI) were registered, in which IBR, VD, RSI viruses played a dominant role and which in 100% of cases were complicated by bacterial pathogens Pasteurella multocida, Neisseria spp., Mycoplasma spp., Candida albicans and others. There is a tendency to increase the etiological role of pathogens IBR and RSI in the structure of viral pneumoenteritis. Field isolates of IBR and PI-3 viruses with an infectious activity of 4.1 lg TCD50 / cm3 (4 passages) and 4.4 lg TCD 50 / cm3 (7 passages), respectively, were isolated from the association and adapted to the transplanted culture of LEK cells. Their biological properties (reproduction on different cell cultures, hemagglutinating and hemadsorbing properties, influence of multiplicity of infection) are studied. The most suitable cell cultures for isolation and subsequent reproduction of infectious rhinotracheitis and parainfluenza-3 from viral associations are cow embryonic lungs (CEL) and calf kidney (CK), the manifestation of CPI on which was observed for 1-3 days of cultivation. Based on the results of this work, a monitoring system for associated pneumoenteritis of cattle of viral etiology was developed, which is presented in the form of scientific and methodological recommendations "Monitoring of associated pneumoenteritis in cattle"

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Golovko Valeryi O
Keleberda Mykola I.
Kolchyk Olena V
Oleshko Andrij Yu.
Solodiankin Oleksii S
Stegnyi Maryna Yu.
Filatov Serhiy V

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2021-03-17