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Registration Number

0221U101200, 0118U003207 , R & D reports

Title

The study of mechanisms of therapy effects of bone marrow mesenchymal stromal cells at acute radiation syndrome and radiation damage of skin

Head

Uzlenkova Nataliia Ye., Кандидат біологічних наук

Registration Date

17-01-2021

Organization

State Organization "Grigoriev Institute for Medical Radiologiy and Oncology of the National Academy of Medical Sciences of Ukraine"

Description

Object of research: rat’s bone marrow mesenchymal stromal cells. Purpose: to define the effectiveness and mechanisms of the therapeutic effect of bone marrow mesenchymal stromal cells (BM MSC) in the treatment of radiation injuries in an experiment. Methods: radiobiological, hematological, cell culture, morphological, statistical. Equipment: CLINAC 6 MeV linear accelerator, UNIDOS universal dosimeter, MN 120 lamina shafts, EC-160 CO2 incubator, microscope Leica 6000B LED, chambers Leіca DFC 310 FX, microscope Leіca DM ІL LED, microscope Axіolab 94D 2601, analytical scales. Theoretical and practical results: it was established therapeutic efficacy of cultured ex vivo BM MSC (1,3 x 106 cells / animal) in allogenic transplantation in an experiment in animals irradiated at a sublethal dose of 5,5 Gy. It was found that in hematopoietic forms of acute radiation syndrome the mechanisms of therapeutic action of allogenic BM MSC are realized by preventing leukopenia at day 2, increasing the number of leukocytes by 3,7 times, lymphocytes by 3,4 times, restoring platelet counts and normalizing the total number of myelocytes at 15 and 22 days, which ensures 100 % survival of the animals. It was found that in acute radiation skin lesions in rats the therapeutic effect of allogenic BM MSC is realized in reducing local inflammation and severity of clinical reactions in the affected area by 1,3 times, reducing the affected areas by 1,9 times and accelerating skin healing during at days 54 after irradiation. Novelty: for the first time in radiobiological experiment in vivo the therapeutic efficacy of allogeneic mesenchymal bone marrow stromal cells in acute radiation syndrome and radiation skin lesions in animals (rats) was evaluated. Use area: radiobiology. 

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Kryvko Alevtyna I
Leonova Iryna O
Mamatkulova Tetiana M.
Maslennikova Olena L
Neniukova Olena V
Pavlichenko Yuliana V.
Skorobogatova Nataliya G