Registration Number
0221U101352,
0116U000260
, R & D reports
Title
Study molecular and biological mechanisms of infectious process at slow infections of cattle
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Head
Gerilovych Anton P., Доктор ветеринарних наук
Gorbatenco Stanislav K, к.вет.н.
Registration Date
20-01-2021
Organization
National Scientific Center Institute of Experimental and Clinical Medicine
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The object of study - minor infections - spread, integration of genetic material into cell culture, the properties of pathogens.
The aim of the work is to study the spread of minor infections in animal husbandry of the world and Ukraine, genetic features and properties of pathogens of immunodeficiency and spimavirus infection, the possibility of integration of field isolates into cell cultures, properties of pathogens at hematological and biochemical levels using a laboratory model.
Research methods - exploratory, epizootological, molecular genetic, hematological, biochemical and statistical.
According to the results of exploratory analysis, the level of prevalence of minor infections, pathogens BLV, BIV and BFV, in the world's livestock. Sampling of blood samples from animals of individual farms in 11 regions of Ukraine revealed genetic material of pathogens of slow infections both in associative forms and in the form of monoinfections. The genetic features of pathogens, the possibility of integration of genetic material into homologous for cattle cultures of LEK and KST cells have been studied. The terms of accumulation of viral mass in cell cultures and in rabbits after subcutaneous inoculation of genetic material have been established. Based on hematological and biochemical studies, the immunosuppressive role of the causative agent of spumavirus infection in rabbits - an increase in the ratio of lymphocytes (80-88%) and total protein by 9.4%, a decrease in CEC by 8.6%, an increase in seromucoids - by 21 , 2% compared with the corresponding indicators of the control group.
Product Description
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Zdanevych Petro P.
Kovalenko Larysa V.
Kuznietsova Olena V.
Lymanska Olga Yu.
Miahkykh Nina V.
Solodjankin Oleksij S.
Stegnyi Maryna Yu.
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2021-01-20
