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Registration Number

0221U102005, 0120U105172 , R & D reports

Title

Functionalization of metal SERS-substrates with biomolecules

Head

Pirko Yaroslav V., Кандидат біологічних наук

Registration Date

27-01-2021

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Description

 During the investigation, the effective ligands for surface modification of SERS substrates compatible with antibodies and other biomolecules were analyzed and selected. The selection of specific antibodies and aptamers to certain specific targets for their further detection were conducted out. An effective method of biofunctionalization of the surface of SERS-substrates by antibodies is also proposed and tested using a specific antibody to lipopolysaccharides (LPS) Salmonella typhimurium (anti-S-Ab). It was found that incubation of the modified surface with anti-S-Ab at concentrations of 1:100 and 1:200 leads to a shift of the photoluminescence peak from 4 to 8 nm, and this effect becomes more intense after incubation of the surface with anti-S-Ab at higher concentrations (1:50 and 1:10). Also, a new and simple method for toxin detection using aptomers, in particular of mycotoxin ochratoxin A using a SERS substrate functionalized with a surface-specific aptamer, has been proposed and tested. As a result of the analysis the characteristic Raman spectrum signals typical for oligonucleotides were obtained. It was found that the detected peaks were located in the region to 300 cm−1 which corresponds to the connection of gold with sulfur and indicates the attachment of the molecule to the surface. The developed sensor is insensitive to non-specific molecules (BSA) which indicates a high selectivity of detection due to the use of a specific aptamer.

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popup.authors

Buziashvili Anastasiya Yu.
Pirko Yaroslav V.
Plokhovska Svitlana H.