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Registration Number

0221U102068, 0115U003744 , R & D reports

Title

Studies of mammalian translation elongation factors in protein biosynthesis and other cell processes.

Head

Negrutskii Boris S, д.б.н.

Registration Date

28-01-2021

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

Description

 The objects of study are a complex of human translation factors 1B, isoforms of translation elongation factor eEF1A, translation elongation factors eEF1Bα, eEF1Bβ, eEF1Bγ, microRNA 663 and 744, Sgt1 protein, actin. The aim of this work is to study the factors of translation elongation in mammals in protein biosynthesis and other cellular processes. Research methods - crystallographic analysis of proteins, cultivation of human cells, tests for the rate of proliferation of tan cells, rate of healing of the "wound surface, confocal fluorescence microscopy, chromatographic isolation of individual proteins, analytical ultracentrifugation, analytical gel filtration, Western blot, co-immunoprecipitation, spivprecipitation protein partners, subcellular fractionation, gel electrophoresis under conditions that do not denature or denature proteins, mass spectrometry, polymerase chain reaction, molecular biological methods of working with DNA, site-directed mutagenesis of mRNA and proteins, kinetic methods, bioinformatic analysis, molecular modeling and molecular docking. Completed tasks of the project: 1. MicroRNA - mediated regulation of expression of translation elongation factor A1 and A2 isoforms in cancer cells was investigated. 2. A model of the factor-mediated mechanism of nucleotide exchange based on the crystal structure of mammalian eEF1A2 has been developed. 3. New eEF1A partner proteins have been identified and the possible functional significance of these interactions has been proposed. 4. The structural organization of the eEF1B subunit of the eEF1B translation elongation factor complex has been studied. 5. New protein-protein interactions of the eEF1H complex in the cytoplasm and nucleus of human cells have been identified and their involvement in certain processes not associated with protein synthesis has been proposed.

Product Description

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Bondarchuk Tetyana V
Lysetskyi Igor L.
Luk`yanenko Ganna I
Novosylna Oleksandra V.
Porubleva Larysa V.
Shalak Viacheslav F