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Information × Registration Number 0221U102450, 0120U103332 , R & D reports Title Development of the technology for obtaining of recombinant bioconjugates for regenerative medicine and study of their properties popup.stage_title Head Kordyum Vitaliy A., Доктор біологічних наук Registration Date 03-02-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  During the first stage of project we have constructed plasmid vectors and developed strains of Escherichia coli for the production of a chimeric protein based on human stromal cell factor- 1ɑ with C-terminal polyhistidine tag, as well as a recombinant analogue of the secretory form of human heparin-binding epidermal growth factor, which contains additional N- and/or C-terminal marker sequences for subsequent chromatographic purification. A metal affinity media for chromatographic purification of His-tag-modified proteins have been synthetized. The developed media exceeds commercial Ni-NTA-Sepharose in terms of stability and efficiency of purification of His-tag-containing proteins. Product Description popup.authors Irodov Dmytro M. Gorbatiuk Oksana B. Dubey Igor Ya. Dubey Larisa V. Kolibo Denis V. Krynina Olha I. Kuziv Yaroslav B. Negrutska Valentina V Pokholenko Yanina O. Rymar Svitlana Iu. Siromolot Andrii A. Toporova Olena K. popup.nrat_date 2021-02-03 Close