Registration Number
0221U102450,
0120U103332
, R & D reports
Title
Development of the technology for obtaining of recombinant bioconjugates for regenerative medicine and study of their properties
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Head
Kordyum Vitaliy A., Доктор біологічних наук
Registration Date
03-02-2021
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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During the first stage of project we have constructed plasmid vectors and developed strains of Escherichia coli for the production of a chimeric protein based on human stromal cell factor- 1ɑ with C-terminal polyhistidine tag, as well as a recombinant analogue of the secretory form of human heparin-binding epidermal growth factor, which contains additional N- and/or C-terminal marker sequences for subsequent chromatographic purification. A metal affinity media for chromatographic purification of His-tag-modified proteins have been synthetized. The developed media exceeds commercial Ni-NTA-Sepharose in terms of stability and efficiency of purification of His-tag-containing proteins.
Product Description
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Irodov Dmytro M.
Gorbatiuk Oksana B.
Dubey Igor Ya.
Dubey Larisa V.
Kolibo Denis V.
Krynina Olha I.
Kuziv Yaroslav B.
Negrutska Valentina V
Pokholenko Yanina O.
Rymar Svitlana Iu.
Siromolot Andrii A.
Toporova Olena K.
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2021-02-03
