Registration Number
0221U102653,
0120U102501
, R & D reports
Title
Regulators of DNA processing systems based on nano-sized metaloorganic complexes of d-metals as potential therapeutic agents for treat various types of diseases
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Head
Govorun Dmitro M., Доктор біологічних наук
Registration Date
08-01-2021
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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Report about Scientific research work: 75 pages, 21 figures, 7 tables, 74 sources.
Complex compounds Pd, Pt, DNA, transcription, replication, transcription, molecular docking, molecular dynamics, test systems - Allium сера.
Object of research - design and synthesis of new organometallic complex compounds Pd, Pt - the mechanism of action of which is to block DNA-containing enzymatic systems of the pathogen.
The purpose of the work – synthesis of Pd, Pt complexes with a flat-square form of coordination node (similar to cisplatin) and replacement of NH3 molecules with 1-allyl-3- (2-hydroxyethyl) thiourea, N-allylmorpholine-4-carbothioamide and 1-allyl-3-tert-butyl compounds for binding to DNA, inhibition of RNA and DNA synthesis in model systems of transcription and replication and study of their cytotoxicity and genotoxicity using a plant test system - Allium sulfur using cisplatin as a comparison drug.
Research methods - chemical synthesis of coordination compounds, X-ray diffraction analysis, and a set of spectral methods for determining and confirming their structure; molecular biological, enzymatic methods of RNA and DNA synthesis.
Results - new π-coordination complexes Pd (II) and Pt (II) with organic ligands - substituted by thiourea were synthesized. They are analogues of the well-known antitumor drug cisplatin
Conclusions - the similarity of the mechanism and effectiveness of the biological effects of synthesized organometallic complexes and cisplatin on model molecular biological and cellular systems are the justification for further research to identify their potential antiproliferative activity and spectrum of antimicrobial action.
Product Description
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Kolomiiets Iryna M.
Palchykovska Larysa G.
Platonov Maksym O.
Stepaniuhin Andrii V.
Shyryna Tetyana V.
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2021-02-08
